Gut Homogenate Extracts
Gut Homogenate Extracts - Related Videos
15:27
Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
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Cited by 18 •
2007
This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.
01:23
Test for Homogeneity
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2023
The goodness–of–fit test can be used to decide whether a population fits a given distribution, but it will not suffice to decide whether two populations follow the same unknown distribution. A different test, called the test for homogeneity, can be used to conclude whether two populations have the same distribution. To calculate the test statistic for a test for homogeneity, follow the same procedure as with the test of independence. The hypotheses for the test for homogeneity can be stated as...
02:47
Evaluating the Effect of Beneficial Bacteria on Gut Microbial Translocation in Heat-Stressed Rats
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2025
Source: Sorokulova, I., et al. Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain. J. Vis. Exp. (2016)This video demonstrates rats pre-treated with beneficial bacteria or phosphate-buffered saline (PBS) and exposed to heat stress to evaluate gut barrier integrity. Fewer gut-derived bacterial colonies from the liver of treated rats, in contrast to numerous colonies in control rats, indicate an intact epithelial barrier and minimal bacterial translocation.
01:27
Oral Infection Alters the Gut Microbiota in a Rat Model
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2026
Begin with a rat model with sutures tied around its upper molar teeth to support bacterial colonization.Repeatedly smear the sutures with a gum disease-causing oral pathogenic bacterium using a gel-like solution.The bacteria colonize the gum around the sutures.Colonization triggers inflammation and damages the tissues.The bacteria release toxins that diffuse through damaged tissues and enter the bloodstream.These circulating toxins reach the gut, cause inflammation, disrupt the epithelial...
07:54
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
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Cited by 9 •
2017
Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model.
FAQs
These preparations can support measurement of cytokines and antibodies, along with microbial products, pathogen burden, and inflammation-associated signals. The combination allows investigators to examine both host responses and infection-related features within intestinal tissue and contents, rather than focusing on only one category of signal.
Filtration or centrifugation separates tissue debris from the extract, creating a preparation better matched to downstream analysis. This step is important because the resulting material can be used in biochemical, immunological, or microbiological assays. The separation therefore links physical processing of the gut sample to the type of measurement planned.
Their value comes from retaining access to multiple biological categories in one intestinal preparation. Investigators can examine host-associated markers such as cytokines, antibodies, and inflammation-associated signals alongside microbial products or pathogen burden. This combined perspective helps connect mucosal immune activity with infection-related changes in the gut environment.
The workflow starts by disrupting intestinal tissue and contents in a buffered solution. The resulting homogenate can then undergo filtration or centrifugation to separate tissue debris from the extract. The prepared material is subsequently directed toward biochemical, immunological, or microbiological assays according to the biological feature under investigation.
They are useful when a study needs information about the intestinal environment during host-microbe interactions, mucosal immune responses, or infection. The extracts can also support investigations of infection severity and treatment effects by enabling analysis of immune markers, microbial signals, pathogen burden, and inflammation-associated changes.
Analysis can quantify cytokines, antibodies, microbial products, pathogen burden, and inflammation-associated signals within the gut. These measurements provide a practical snapshot of intestinal conditions and can help characterize mucosal immune responses, evaluate infection-related changes, or examine how treatment affects the biological environment.