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A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
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A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

DOI:
10.3791/56053-v

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07:54 min

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July 25, 2017

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, , , ,
1Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA),Consejo Superior de Investigaciones Científicas (CSIC), 2Department of Food Safety and Infection Biology,Norwegian University of Life Sciences (NMBU), 3Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria,Universidad Complutense de Madrid (UCM), 4Faculty of Chemistry, Biotechnology and Food Science,Norwegian University of Life Sciences (NMBU)

Chapters

  • 00:05Title
  • 00:40Preparation of Bacterial Cultures to Inoculate Mice
  • 01:46Mice Assay and Experimental Design and Collecting Samples
  • 03:08LAB Counting and Bacteriocin Activity
  • 04:39DNA Extraction, 16S rDNA Amplification, and Sequencing
  • 05:45Results: Detection and Comparison of Bacteriocin Production in Lactic Acid Bacteria Recovered from Feces
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model.

Tags

Keywords: Gut MicrobiotaBacteriocinsMouse ModelProbioticBacterial PopulationsPhysiological ParametersHost OrganismSerial DilutionsColony Forming UnitsDrinking WaterBacterial SurvivalBALB/c MiceFecal SamplesBacterial SuspensionBacteriocin-producing BacteriaMRS Soft Agar

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