Immune Profiling
Immune Profiling - Related Videos
01:38
What is the Immune System?
0 Views •
2019
Overview The immune system comprises diverse biological structures and processes that protect the body from disease. These processes can be classified into innate and adaptive immunity. To work effectively, the immune system needs to detect pathogens by distinguishing the body’s own structures from foreign elements. If this determination fails, autoimmune diseases occur in which the immune system reacts against the body’s own tissue. The Innate Immune System Acts Fast and Non-specifically
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
0 Views •
Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
08:29
Expression Profiling with Microarrays
0 Views •
2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
0 Views •
Cited by 30 •
2016
We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.
01:36
Humoral Immune Responses
0 Views •
2019
Overview The humoral immune response, also known as the antibody-mediated immune response, targets pathogens circulating in “humors,” or extracellular fluids, such as blood and lymph. Antibodies target invading pathogens for destruction via multiple defense mechanisms, including neutralization, opsonization, and activation of the complement system. Patients that are impaired in the production of antibodies suffer from severe and frequent infections by common pathogens and unusual pathogens. B...
FAQs
Immune profiling becomes more informative when cellular composition, signaling molecules, and functional responses are interpreted together rather than as isolated measurements. Cell populations show which immune components are present, marker expression indicates immune state, and stimulus-response testing reveals activity. Integrated patterns can therefore separate protective immunity from dysregulated responses in infection research.
Marker measurements describe the immune state, but functional testing shows how that system responds when challenged by a specific stimulus or infection. Combining these perspectives helps researchers determine whether an observed cell population or signaling pattern is associated with effective activity, limited activity, or dysregulation, improving interpretation of immune status.
Flow cytometry identifies immune cell populations and measures marker expression at the cellular level. Cytokine assays assess signaling molecules released or present in the sample, while immune-receptor or gene-expression sequencing characterizes receptor or expression patterns. Using these approaches together provides broader coverage than relying on any single measurement type.
A typical investigation begins with a biological sample and a question about immune status, activity, infection, or treatment response. Researchers then characterize cell populations and marker expression, measure signaling molecules, and assess responses to selected stimuli when appropriate. Sequencing-based measurements can add receptor or gene-expression information for integrated analysis.
The approach is useful when researchers need to follow disease progression, examine host-pathogen interactions, or determine whether an immune response is protective or dysregulated. It can also compare immune states across conditions and reveal changes associated with infection. These applications help connect observed immune patterns with disease mechanisms and outcomes.
Immune profiling can track how vaccination or treatment changes immune cell populations, marker expression, signaling molecules, and functional responses. Comparing profiles before and after an intervention may show whether the intended immune activity occurred and whether responses shifted toward protection or dysregulation. The resulting patterns can also support identification of diagnostic or prognostic biomarkers.