Laser Illumination
Laser Illumination - Related Videos
07:07
Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion
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Cited by 13 •
2013
Here we describe a protocol for optogenetic manipulation of motoneuronal activity while monitoring changes in motor output (muscle contraction) in semi-intact Drosophila larvae using lasers within a conventional confocal microscope. This technique enables researchers to achieve local perturbation of neural activity in a few neuromeres to elucidate the dynamics of motor circuits.
04:09
Modeling Photothrombotic Stroke in Mouse Model: A Laser Illumination Technique Through Mouse Skull Following Photosensitive Dye Administration to Induce Photothrombosis
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2025
This video demonstrates the procedure to generate photothrombotic stroke in a mouse model by laser illumination of an intact skull following administration of photosensitive dye.
10:07
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
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2014
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
07:24
Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose Bengal
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2025
This manuscript presents a novel, innovative 3D-printed illumination device for studying Rose Bengal-mediated photodynamic therapy in vitro.
11:15
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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Cited by 114 •
2016
This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.
FAQs
Wavelength selection determines how effectively a laser excites a fluorescent label attached to or associated with the biological material. The resulting emitted light can then reveal structures, signaling events, microbial components, or host-pathogen interactions. Choosing suitable illumination conditions helps the system distinguish the fluorescence signal needed for a particular immunology or infection experiment.
Laser intensity and timing control how strongly and when biological signals are observed. Adjusting these parameters allows researchers to examine changes with spatial and temporal precision, such as immune-cell signaling or pathogen behavior. Their control is important for comparing experimental conditions and for measuring infection-related changes as they develop.
A focused beam directs excitation to the selected biological material, allowing emitted light to be associated with particular cells, proteins, microbial components, or interactions. This focused approach supports detailed visualization and measurement rather than relying only on broad illumination. In immunology and infection studies, it helps resolve localized changes within complex biological samples.
Fluorescence microscopy uses laser illumination within an imaging system to examine labeled structures and interactions with spatial detail. Flow cytometry uses laser excitation to support detection and quantification of labeled cells or cellular features as part of a measurement system. The choice depends on whether the experiment emphasizes visual location or cell-level measurement.
A typical workflow begins by selecting fluorescent labels relevant to the cells, proteins, microbial components, or interaction being studied. The instrument then applies controlled laser wavelength, intensity, and timing, collects emitted light, and uses the resulting signal for visualization or quantification. Researchers can compare these measurements across immune or infection-related conditions.
Laser-based fluorescence measurements can help characterize immune responses, track pathogen behavior, and examine interactions between hosts and pathogens. They can also support evaluation of diagnostic or therapeutic strategies by revealing or quantifying infection-related changes. The resulting data may describe cellular structures, signaling events, microbial components, or changes detected in labeled samples.