Live Mice
Live Mice - Related Videos
07:10
Femoral Bone Marrow Aspiration in Live Mice
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Cited by 18 •
2014
This protocol describes a procedure for serial sampling of femoral bone marrow (BM) without requiring the sacrifice of mice. This procedure facilitates longitudinal studies of the BM composition of mice over time and provides serial access to cells within the BM for ex vivo and transplantation studies.
08:23
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
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Cited by 18 •
2013
Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. In this article, we present a detailed method for imaging the dynamics of subcellular structures, such as the secretory granules, in the salivary glands of live mice.
08:05
Doppler Ultrasonography for Live Imaging and Quantification of Ovarian Vascular Function in Mice
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Cited by 1 •
2025
Changes in ovarian blood flow during the preovulatory period are essential for ovulation. Doppler ultrasonography has been used in humans and large animals to assess ovarian hemodynamics and perfusion. A protocol is presented for applying Doppler ultrasonography to evaluate ovarian hemodynamics during the preovulatory and periovulatory phases in mice.
09:01
Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice
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Cited by 9 •
2015
We describe methods for longitudinal monitoring of the efficacy of therapeutics for the treatment of colonic pathologies in mice using a rigid endoscope. This protocol can be readily used for the characterization of the therapeutic response of an individual tumor in live mice and also for monitoring potential disease relapse.
09:34
Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice
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Cited by 27 •
2019
This method describes a chronic preparation that allows optical access to the hippocampus of living mice. This preparation can be used to perform longitudinal optical imaging of neuronal structural plasticity and activity-evoked cellular plasticity over a period of several weeks.
FAQs
Live mice preserve interactions among pathogens, immune cells, organs, and host physiology within an intact organism. This allows investigators to observe disease progression and treatment responses as connected biological events, rather than examining isolated components separately. The resulting context is especially useful when infection or immune protection depends on coordinated activity across tissues and over time.
By monitoring responses over time, investigators can follow how innate and adaptive immunity develop after exposure to a pathogen, vaccine, or immune-modulating treatment. This temporal view connects immune activity at different stages with later immune protection or disease progression. In immunology and infection studies, that progression provides an important outcome that isolated systems cannot fully reproduce.
Tissues and organs provide the locations in which immune responses and infection-related processes can be observed within the animal. Monitoring across these sites, together with host physiology, reveals system-wide interactions that isolated cell systems cannot fully reproduce. Such observations connect pathogen activity with immune and physiological responses across the organism.
Different interventions create distinct experimental contexts: pathogen exposure addresses infection mechanisms, vaccination tests immune protection, and immune-modulating treatment examines altered responses and treatment effects. By monitoring these conditions in live mice, investigators can compare disease progression or immune outcomes within the same intact biological setting.
A basic workflow begins with a defined intervention, such as pathogen exposure, vaccination, or administration of an immune-modulating treatment. Investigators then monitor responses and disease progression over time, including changes across tissues. Experimental design and ethical oversight are needed to ensure that the study is scientifically justified and conducted humanely.
These studies can support research on infection mechanisms, vaccine efficacy, inflammation, antimicrobial therapies, and immune protection. Their value comes from linking an intervention with responses throughout an intact organism, so investigators can examine both biological effects and disease progression. Findings may therefore inform how treatments or vaccines perform in host contexts.
Ethical oversight is closely connected to scientific design in live-mouse research. Studies should be structured so animal use is scientifically justified, observations address the research question, and procedures remain humane. This requirement supports responsible investigation of infection, immunity, and treatment responses while recognizing that the model uses living organisms.