Lung Slice Preparation
Lung Slice Preparation - Related Videos
04:26
Agarose-Based Preparation of Human Precision-Cut Lung Slices
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2025
In this video, we describe a procedure to inflate isolated human lungs with agarose and prepare human precision-cut lung slices. The prepared ex vivo lung slices can be used for further immunologic experiments.
08:01
Engineered Lung Tissues Prepared from Decellularized Lung Slices
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Cited by 7 •
2022
This protocol describes a method to generate reproducible, small-scale engineered lung tissues, by repopulating decellularized precision-cut lung slices with alveolar epithelial type 2 cells, fibroblasts, and endothelial cells.
05:33
Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices
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2023
Organotypic primary tissue explant cultures, which include precision-cut slices, represent the three-dimensional (3-D) tissue architecture as well as the multicellular interactions of native tissue. This video describes the culturing of murine lung tumor-derived tissue slices using a rotating incubation unit, a system that enables intermittent exposure of tissues to oxygen and nutrients. The protocol describes the methodology to generate and culture precision-cut lung tumor slices for drug...
09:33
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
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Cited by 52 •
2017
We describe a method for generating Precision-cut Lung Slices (PCLS) and immunostaining them to visualize the localization of various immune cell types in the lung. Our protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.
08:12
Thin Sectioning of Slice Preparations for Immunohistochemistry
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Cited by 7 •
2007
The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.
FAQs
Preserving the three-dimensional relationships among airways, alveoli, immune cells, and extracellular matrix keeps respiratory events tied to their tissue context. This allows investigators to examine cellular interactions and structural features together, rather than interpreting cells in isolation. In immunology and infection studies, that context supports more physiologically relevant observation of pathogen invasion and inflammatory responses.
Stabilization and controlled sectioning help retain key structural features while keeping the tissue suitable for ex vivo analysis. These steps determine whether airways, alveoli, immune cells, and extracellular matrix remain positioned for meaningful observation. Consistent processing therefore supports direct imaging and controlled experiments in which researchers can examine biological responses within intact lung tissue.
Lung slices occupy an intermediate experimental position. Unlike isolated cell cultures, they preserve relationships among multiple lung structures and cell types. Unlike animal studies, they provide tissue for direct imaging and controlled testing outside the whole organism. This combination allows researchers to investigate respiratory biology in a more relevant tissue setting while retaining experimental control over tested conditions.
The tissue context enables investigation of pathogen invasion, inflammatory responses, and immune-cell activity within lung structures. Researchers can observe how these processes relate to airways, alveoli, and extracellular matrix rather than assessing them separately. Lung slices can also support evaluation of treatment effects, helping connect immune or infectious changes with visible responses in the surrounding tissue.
The workflow proceeds through collection of lung tissue, stabilization of the sample, and sectioning under controlled conditions. Each stage supports the next: collected tissue must remain suitable for processing, stabilization helps preserve relevant features, and controlled sectioning produces material appropriate for ex vivo analysis. The resulting slices can then be used for imaging or controlled experimental testing.
Researchers would select this approach when they need to study immune or infectious events in preserved lung tissue rather than in isolated cells alone. It is useful for examining pathogen invasion, inflammatory responses, immune-cell activity, and treatment effects through direct imaging and controlled testing. In this role, lung slices complement cell cultures and animal studies by adding physiologically relevant tissue context.