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Engineered Lung Tissues Prepared from Decellularized Lung Slices
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Bioengineering
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JoVE Journal Bioengineering
Engineered Lung Tissues Prepared from Decellularized Lung Slices

Engineered Lung Tissues Prepared from Decellularized Lung Slices

DOI:
10.3791/63151-v

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08:01 min

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January 21, 2022

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, , ,
1Department of Biomedical Engineering,Yale University, 2Yale School of Medicine, 3Department of Cellular and Molecular Physiology,Yale School of Medicine, 4Department of Anesthesiology,Yale School of Medicine

Chapters

  • 00:04Introduction
  • 00:35Harvest of Rat Lungs
  • 01:15Cutting and Clipping the Lung Slices
  • 02:25Slice Decellularization
  • 03:35Slice Recellularization and Culture
  • 06:20Results: Visualization of the Structure of Decellularized Lung Scaffolds and Recellularized Engineered Lung Tissues (ELTs)
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method to generate reproducible, small-scale engineered lung tissues, by repopulating decellularized precision-cut lung slices with alveolar epithelial type 2 cells, fibroblasts, and endothelial cells.

Tags

Extracellular MatrixLung SliceDecellularizationCassetteAgaroseHBSSPBSRazor BladeOrbital Shaker

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