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JoVE Journal
Immunology and Infection

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Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
 

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Article doi: 10.3791/55465
April 5th, 2017

Summary April 5th, 2017

We describe a method for generating Precision-cut Lung Slices (PCLS) and immunostaining them to visualize the localization of various immune cell types in the lung. Our protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.

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