Murine Lung Tumors
Murine Lung Tumors - Related Videos
04:28
Lung Tumor Cell Recruitment Assay
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2019
This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis.
05:33
Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices
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2023
Organotypic primary tissue explant cultures, which include precision-cut slices, represent the three-dimensional (3-D) tissue architecture as well as the multicellular interactions of native tissue. This video describes the culturing of murine lung tumor-derived tissue slices using a rotating incubation unit, a system that enables intermittent exposure of tissues to oxygen and nutrients. The protocol describes the methodology to generate and culture precision-cut lung tumor slices for drug...
06:31
Primary Tumor and MEF Cell Isolation to Study Lung Metastasis
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Cited by 13 •
2015
The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.
07:31
Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs
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Cited by 13 •
2016
Metastasis plays a profound role in the virulence of cancer, accounting for an estimated 90% of deaths. We report a protocol for a metastatic melanoma model in mice that is useful for determining the efficacy of therapeutic agents against this clinical phenomenon.
08:17
Dynamic Lung Tumor Tracking for Stereotactic Ablative Body Radiation Therapy
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Cited by 9 •
2015
Stereotactic ablative body radiation therapy (SBRT) involves the precise delivery of high-dose radiation to cancer tumor targets. A novel SBRT platform offers a first-of-its-kind gimbaled radiation accelerator mounted within a pivoting O-ring gantry capable of dynamic image-guided tumor tracking. Here, we describe dynamic tumor tracking for lung targets.
FAQs
These cell populations communicate through inflammatory mediators, immune-cell recruitment, and immune-surveillance pathways. Such interactions can alter the local tumor environment and shape how tumors begin, progress, or respond to treatment. Examining the communication among these compartments helps investigators separate effects driven by tumor cells from effects arising through the surrounding host response.
Genetic or environmental changes can disrupt regulated cell growth, influencing whether lung tumors arise and how they develop afterward. Studying these influences gives researchers a way to connect altered growth control with changes in tumor initiation and progression. The approach also supports analysis of how host conditions modify cancer biology rather than treating tumors as isolated cell populations.
Inflammation can change the conditions surrounding a developing tumor, while immune-surveillance pathways determine how the host recognizes and responds to abnormal cells. Infectious challenges may further alter these processes. Investigators therefore use murine lung tumors to examine how inflammatory signals and host immunity affect tumor initiation, progression, and sensitivity to treatment.
These models allow investigators to study treatment responses in the presence of tumor cells and their surrounding stromal and immune compartments. Because immune-cell recruitment and surveillance pathways can influence outcomes, researchers can examine how immunotherapies interact with host immunity. The resulting observations support investigation of immune mechanisms associated with therapeutic response.
They can be used to investigate how infectious challenges or inflammation alter cancer-related processes, including tumor initiation, progression, and treatment response. This connects infection biology with the immune regulation of cancer in a controlled experimental setting. The models therefore support questions that require examining both neoplastic changes and the host response to environmental or infectious influences.
Their experimental tractability enables investigators to identify immune mechanisms that may help explain lung-cancer behavior or treatment response. These findings do not replace human research, but they provide a model for connecting tumor biology with host immunity before mechanisms are considered in the context of human disease. Their value lies especially in studying interactions that are difficult to isolate clinically.