Skin Inflammation Model
Skin Inflammation Model - Related Videos
03:46
Malassezia-Induced Skin Inflammation and Fungal Burden Analysis in a Murine Model
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2025
This video demonstrates Malassezia-induced skin infection in mice. Malassezia oil suspension is applied over the wounded ear, triggering inflammation and swelling of the ear tissue. Later, the ears are dissected, homogenized, and spread on an agar plate for growth. The number of colonies indicates the fungal burden resulting from skin inflammation.
02:49
A Technique to Assess the Immune Response against an Antigen by Inducing Skin Inflammation
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2025
This video demonstrates an in vivo technique to assess a test antigen by inducing skin inflammation in a murine model. The test antigen—a hapten in an oil/acetone solution—is topically applied to the skin of the mouse ear, and inflammation of the skin is assessed after an adequate period.
03:06
A Method to Induce Ocular Surface Inflammation in a Murine Model
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2025
This video demonstrates a method to induce ocular surface inflammation and meibomian gland dysfunction in a murine model. Stimulation of the mouse's immune system using an immunogen and later restimulation with the same immunogen promotes ocular surface inflammation, which causes plugging of meibomian gland secretion through neutrophil-produced aggNETs and leads to dry eye disease.
08:02
Th17 Inflammation Model of Oropharyngeal Candidiasis in Immunodeficient Mice
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Cited by 11 •
2015
Although Candida infection models are available to study host immune resistance, a model to study T cell mediated immunopathology in the context of Candida infection is absent. Here we describe a method to establish Th17 immunopathology associated with oral Candida infection in immunodeficient mice.
06:30
An ex vivo Mouse Model for High-Fat Diet-Like Adipose Tissue Inflammation
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2026
Using an ex vivo explant model, small pieces of the rostral portion of visceral white adipose tissue from lean mice are cultured for 7–10 days, enabling the induction and study of adipose tissue inflammation as it occurs during high-fat diet feeding.
FAQs
Defined inflammatory stimuli activate signaling pathways in skin cells or tissue, leading to cytokine and chemokine release. These molecular signals can coincide with barrier disruption and altered immune-cell behavior. Measuring these linked responses helps investigators examine how an initial stimulus develops into a broader inflammatory reaction relevant to skin disease or infection.
These model components provide different biological settings for examining skin inflammation. Cultured keratinocytes allow responses from skin cells to be studied directly, reconstructed skin represents a more organized skin system, and animal tissue supplies tissue-level responses. Comparing these settings can show whether findings remain consistent across increasingly complex experimental systems.
Inflammation can contribute to host defense while also causing harmful tissue effects. A Skin Inflammation Model helps examine this balance by linking immune responses with cytokine and chemokine release, barrier disruption, and changes in immune-cell behavior. This distinction is important when interpreting disease mechanisms and assessing whether a treatment reduces harmful responses without obscuring protective activity.
Investigators expose cultured cells, reconstructed skin, or animal tissue to a selected inflammatory stimulus under controlled experimental conditions. They then examine responses such as signaling-pathway activation, cytokine and chemokine release, barrier changes, or immune-cell behavior. This workflow connects the chosen stimulus with specific features of the resulting inflammatory response.
The model can provide several complementary outcomes rather than a single inflammation measurement. Researchers can assess signaling-pathway activation, the release of cytokines and chemokines, disruption of the skin barrier, and changes in immune-cell behavior. Together, these findings help characterize host responses and indicate whether inflammation appears protective, damaging, or treatment-responsive.
They are useful for examining how skin responds to pathogens or other defined inflammatory challenges and for separating host-defense responses from tissue-damaging inflammation. The controlled setting also supports evaluation of topical anti-inflammatory and antimicrobial therapies. Related applications include research on eczema, psoriasis, and wound healing, where altered inflammation or barrier function is relevant.