Ultra Filtration
Ultra Filtration - Related Videos
02:49
Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device
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2025
This video demonstrates the isolation and concentration of bacterial culture filtrate proteins, which include bacterial extracellular vesicles, using ultrafiltration. The concentrated extracellular vesicles serve as diagnostic markers for disease detection.
10:19
Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
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Cited by 9 •
2016
Design and fabrication of a three-dimensionally (3-D) printed microfluidic cross-flow filtration system is demonstrated. The system is used to test performance and observe fouling of ultrafiltration and nanofiltration (thin film composite) membranes.
09:18
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
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Cited by 22 •
2018
Here, we describe two extracellular vesicle isolation protocols, ultrafiltration centrifugation and ultracentrifugation with density gradient centrifugation, to isolate extracellular vesicles from murine bronchoalveolar lavage fluid samples. The extracellular vesicles derived from murine bronchoalveolar lavage fluid by both methods are quantified and characterized.
10:53
Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
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Cited by 2 •
2023
Virus concentration from environmental water and wastewater samples is a challenging task, carried out primarily for the identification and quantification of viruses. While several virus concentration methods have been developed and tested, we demonstrate here the effectiveness of ultrafiltration and skimmed milk flocculation for RNA viruses with different sample types.
12:47
Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
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Cited by 20 •
2012
Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation.
FAQs
Membrane pore size sets the size threshold for retention, helping determine which biological components remain in the concentrated fraction and which move into the filtrate. Operating conditions also influence separation, so pore size should be considered together with applied pressure and other process conditions. This control allows researchers to tailor processing for proteins, particles, or microorganisms.
Pressure difference supplies the driving force that moves fluid through the semipermeable membrane. Because pressure is an operating condition, it works together with membrane pore size to determine how effectively the sample separates into retained material and filtrate. Managing these conditions supports production of a usable preparation for concentration, purification, or downstream biological analysis.
Ultrafiltration combines size-based retention with passage of smaller solutes through the membrane. Macromolecules, particles, or microorganisms remain in the retained fraction, while substances such as salts and water enter the filtrate. This separation can reduce low-molecular-weight contaminants while preserving the larger material needed for immunoassays, protein analysis, or pathogen characterization.
The same size-selective separation can accomplish two related goals. Retaining larger biological components concentrates them in a smaller product fraction, while passage of water, salts, and other smaller solutes improves sample purity. This dual function is useful when researchers need a more concentrated and cleaner preparation without losing the macromolecules or particles required for later analysis.
A biological sample is processed across a semipermeable membrane under a pressure difference. Antibody-containing macromolecules remain in the retained fraction, while smaller solutes such as salts and water pass into the filtrate. The resulting material can undergo concentration or buffer exchange, improving its suitability for immunoassays and protein analysis.
For pathogen or viral particle enrichment, a sample is processed so larger particles are retained while smaller solutes pass into the filtrate. The resulting fraction can support pathogen characterization, and removal of low-molecular-weight contaminants may improve subsequent analysis. This makes the method useful when particle-focused measurements require cleaner biological material.
Ultrafiltration helps prepare biological samples for several immunology and infection workflows, including antibody concentration, buffer exchange, pathogen or viral particle enrichment, and contaminant removal. By improving sample quality, it supports downstream immunoassays, protein analysis, and pathogen characterization. The method therefore connects sample processing with more reliable examination of immune-related molecules and infectious agents.