Animal Survival Assessment

Animal Survival Assessment is the systematic evaluation of whether an animal remains alive and clinically stable during or after an illness, treatment, experiment, or environmental challenge. It combines defined survival endpoints with observations of physical condition, behavior, activity, and other health indicators, allowing researchers to distinguish recovery, deterioration, and humane endpoint criteria over a specified period. In medicine, this assessment supports preclinical studies by measuring treatment outcomes, disease progression, and intervention safety while helping guide ethical decisions about animal welfare. Standardized assessments improve data quality, enable comparisons among experimental groups, and clarify how well findings may inform further biomedical research.

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JoVE EoE - Assay Techniques

Cellular Impedance-Based Survival Kinetics Assay to Assess Virus Survival

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2025

This video describes a cellular impedance-based technique to study the survival kinetics of viruses that induce cytopathic effects. The method measures the electrical impedance of virus-infected adherent cells pre-treated with saline.

Assessing the Survival of a Bacterial Pathogen in Mouse Peritoneal Macrophages

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2026

Source: Zhang, C. et al. Development and Assessment of Intracellular Infection Models for Staphylococcus aureus. J. Vis. Exp. (2025)This video demonstrates the quantification of intracellular survival of a methicillin-resistant bacterial pathogen in mouse peritoneal macrophages. It outlines the steps involved in infection, extracellular bacterial clearance, host cell lysis, and colony enumeration to assess bacterial survival within host macrophages.

Survivable Stereotaxic Surgery in Rodents

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Cited by 50 •

2008

The monitoring of extracellular neurotransmitter levels in distinct brain regions of freely moving animals offers insights on the link between neurotransmitter release and behavior. In vivo microdialysis coupled with electrochemical detection provides excellent anatomical and chemical resolution; and information on how basal neurotransmission is altered by pharmacological or physiological manipulations.

C. elegans Survival Assay: A Short-Term Toxicity Test

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2023

This video introduces a short-term survival assay that tests a treatment for acute toxicity or tests a genetic strain for resistance to a toxic condition. The example protocol shows an example survival assay in which worms are tested for their ability to resist oxidative stress induced by paraquat (PQ).

Research

JoVE Journal - Medicine
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Incorporation of a Survivable Liver Biopsy Procedure in Mice to Assess Non-alcoholic Steatohepatitis (NASH) Resolution

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Cited by 5 •

2019

In clinical settings, a liver biopsy is used to assess stages of non-alcoholic steatohepatitis (steatosis, inflammation, hepatocyte ballooning, and fibrosis). Here, we illustrate a survivable liver biopsy in mice which can be used for histological analyses to enable assessment of therapeutic agents in a manner more aligned with clinical trials.

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