0 views • 2:56 min • April 30th, 2023
- Begin by preparing the compound to be tested for toxicity, and distribute it to the wells of an assay plate. Transfer worms of the appropriate developmental stage to each well, and immediately start a timer. Note any worms that are damaged and do not include them in your survival calculations.
After a predetermined time, count the number of worms that are still alive in each well. The bright light of a dissection microscope or gentle tapping on the plate should cause any live worms to move. Repeat this observation after each time interval, until most of the animals are dead. In the following protocol, we will observe L4 larvae survival time during exposure to paraquat, a compound that induces oxidative stress.
- In this demonstration, oxidative stress will be induced using paraquat. Prepare a fresh 100 millimolar paraquat solution as described in the accompanying protocol text. Paraquat is a hazardous and highly toxic compound, and must be handled according to appropriate guidelines.
Pipette 40 microliters of the paraquat solution to every well of a 96-well plate. Use at least 12 wells as replicates of every condition tested. Using a platinum wire worm pick, transfer five to eigh
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