Collagen Embedding
Collagen Embedding - Related Videos
07:25
Collagen Hydrogels
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2023
Collagen is another widely used biomaterial that has found popularity in commercial applications, such as photography. Collagen has more recently been used in tissue engineering applications, by creating hydrogels that provide structure to engineered tissue. This video introduces collagen as a biomaterial, demonstrates how it is harvested from porcine skin, and shows how the material is used to create a hydrogel for tissue engineering applications. Finally, several applications of the material...
08:49
Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
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Cited by 6 •
2016
This manuscript describes a soft lithography-based technique to engineer uniform arrays of three-dimensional (3D) epithelial tissues of defined geometry surrounded by extracellular matrix. This method is amenable to a wide variety of cell types and experimental contexts and allows for high-throughput screening of identical replicates.
09:28
Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
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Cited by 9 •
2010
Creation of micro-tissues using cylindrical collagen gels, called modules, that contain embedded cells and which surface is coated with endothelial cells.
07:03
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
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Cited by 18 •
2014
This procedure demonstrates in vivo near IR fluorescence imaging of collagen remodeling activities in mice as well as ex vivo staining of collagens in tissue sections using caged collagen mimetic peptides that can be photo-triggered to hybridize with denatured collagen strands.
10:14
Generation of 3D Collagen-Embedded A549/MRC-5 Alveolar Organoids using a Mechanical Cell-Stretching Bioreactor
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2026
This protocol outlines the generation of organoids from A549 alveolar epithelial cells and MRC-5 lung fibroblasts on an extracellular matrix hydrogel scaffold, followed by embedding in 3D collagen gels and exposure to mechanical strain using a cell-stretching bioreactor to investigate alveolar mechanobiology.
FAQs
Soluble collagen polymerizes into a fibrillar hydrogel only under controlled pH and temperature conditions. These conditions determine whether the matrix forms the intended supportive structure around the embedded specimen. Consistent control is therefore important for producing comparable three-dimensional environments when studying cell behavior, tissue organization, or responses to medical interventions.
The fibrillar matrix provides more than physical support: it creates a surrounding environment with which embedded biological material can interact. Cells may attach to the scaffold, migrate through it, and respond to its three-dimensional organization. This makes collagen embedding useful for examining tissue behavior in a setting that more closely represents structural conditions than a flat culture surface.
Flat culture surfaces provide limited spatial structure, whereas collagen embedding places cells or tissue within a three-dimensional matrix. This difference allows researchers to examine attachment, migration, and interactions with the surrounding scaffold in a more tissue-like context. The resulting model can reveal aspects of biological behavior that are less evident in conventional two-dimensional culture.
A typical workflow begins by preparing soluble collagen and suspending the selected cells, tissue, or specimen within it. The mixture is then exposed to controlled pH and temperature conditions so the collagen polymerizes into a fibrillar hydrogel. After the matrix forms, researchers can observe biological behavior within the three-dimensional environment.
Researchers use the technique when they need to model tissue structure or behavior during wound repair, tissue engineering, disease progression, or therapeutic-response studies. Embedding creates a matrix-based experimental setting in which biological specimens can attach, migrate, and interact with their surroundings, supporting investigations that require more realistic structural context than flat culture alone.
Collagen-embedded models can support evaluation of how cells or tissues behave during repair, disease-related changes, and exposure to therapies. They also contribute to the development of regenerative and diagnostic strategies by connecting cell-level observations with tissue-like organization. In medicine, this helps bridge basic cell biology and the testing of potential clinical approaches.