Electrophoretic Clearing
Electrophoretic Clearing - Related Videos
08:17
Electrophoretic Separation of Proteins
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Cited by 29 •
2008
In this video, we demonstrate a method for electrophoretic separation of proteins using poly-acrylimide gel electrophoresis (PAGE).
06:57
Electrophoretic Mobility Shift Assay (EMSA)
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2023
The electrophoretic mobility shift assay (EMSA) is a biochemical procedure used to elucidate binding between proteins and nucleic acids. In this assay a radiolabeled nucleic acid and test protein are mixed. Binding is determined via gel electrophoresis which separates components based on mass, charge, and conformation. This video shows the concepts of EMSA and a general procedure, including gel and protein preparation, binding, electrophoresis, and detection. Applications covered in this video...
04:17
Electrophoretic Delivery of GABA into the Epileptic Focus of an Anesthetized Mouse
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2025
Source: Slezia, A. et al. Electrophoretic Delivery of γ-aminobutyric Acid (GABA) into Epileptic Focus Prevents Seizures in Mice. J. Vis. Exp. (2019)This video demonstrates the electrophoretic delivery of gamma-aminobutyric acid (GABA) to inhibit seizure activity in an anesthetized mouse. It outlines the steps involved in probe implantation, neural activity recording, seizure induction, and controlled GABA delivery via a microfluidic ion pump to assess its therapeutic efficacy.
10:03
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
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Cited by 11 •
2012
Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.
08:25
Analysis of Coronary Vessels in Cleared Embryonic Hearts
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Cited by 11 •
2016
We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.
FAQs
The electric field drives charged detergents and ions through the specimen, rather than relying only on passive diffusion. This accelerates lipid extraction, which reduces light scattering while the tissue’s structural framework remains available for three-dimensional analysis. In protocols that preserve fluorescent labels, the same cleared specimen can retain molecular signals for later imaging.
Lipid extraction reduces the components that scatter light within biological tissue. Lower scattering allows light to travel through larger specimen volumes, making internal structures more accessible for volumetric microscopy. The benefit is not simply improved appearance: reduced optical obstruction supports analysis of cellular organization, vessels, tumors, and neural tissue throughout three-dimensional specimens.
Refractive-index matching prepares the cleared specimen for optical imaging by pairing it with a suitable refractive-index medium. This step follows removal of light-scattering components and enables the specimen to be examined using light-sheet or confocal microscopy. The resulting combination supports imaging through cleared tissue volumes rather than limiting observation to exposed surfaces or thin sections.
A typical workflow applies an electric field to drive charged detergents and ions through the tissue, accelerating lipid extraction while maintaining the structural framework. After optical clearing, the specimen is matched with a refractive-index medium and examined by light-sheet or confocal microscopy. The sequence connects chemical processing with three-dimensional image acquisition.
Some electrophoretic clearing protocols maintain fluorescent labels during tissue processing. When those signals remain available, researchers can examine labeled structures within the cleared three-dimensional specimen rather than relying only on general tissue architecture. This expands the information obtained from the sample by combining preserved fluorescence with volumetric visualization of organs, tumors, vessels, or neural tissue.
Cleared specimens support volumetric examination of organs, vessels, tumors, and neural tissue. Researchers can use these views to investigate disease architecture, cellular organization, and treatment-related changes across intact tissue volumes. This perspective is especially valuable when the spatial arrangement of structures matters and conventional thin-section histology cannot show the full three-dimensional context.
Thin-section histology examines tissue through separate, limited slices, whereas electrophoretic clearing enables analysis across larger three-dimensional specimen volumes. The approaches therefore answer different spatial questions. Clearing can reveal relationships among cells, vessels, tumors, or neural structures throughout a specimen, while histology remains a section-based view. Together, they can provide complementary information about tissue organization and disease architecture.