Longitudinal In Vivo Imaging
Longitudinal In Vivo Imaging - Related Videos
07:15
In vivo Macrophage Imaging Using MR Targeted Contrast Agent for Longitudinal Evaluation of Septic Arthritis
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Cited by 2 •
2013
We demonstrate how to perform macrophage MR imaging using ultrasmall superparamagnetic contrast agent (USPIO) in septic arthritis, allowing an initial and longitudinal in vivo non-invasive evaluation of macrophages infiltration and an assessment of therapy efficacy.
07:47
Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases
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Cited by 9 •
2016
This manuscript describes a procedure to track the remodeling of the cerebrovasculature during amyloid plaque accumulation in vivo using longitudinal two-photon microscopy. A thinned-skull preparation enables the visualization of fluorescent dyes to assess the progression of cerebrovascular damage in a mouse model of Alzheimer's disease.
10:13
Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)
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Cited by 24 •
2016
Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.
05:54
Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
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Cited by 31 •
2013
A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.
07:44
Longitudinal Intravital Imaging Through Clear Silicone Windows
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Cited by 9 •
2022
An approach is here presented for long-term intravital imaging using optically clear, silicone windows that can be glued directly to the tissue/organ of interest and the skin. These windows are cheaper and more versatile than others currently used in the field, and the surgical insertion causes limited inflammation and distress to the animals.
FAQs
The main advantage is within-subject comparison: each subject serves as its own reference across imaging sessions. This reduces variation caused by differences between separate specimens, making temporal changes easier to attribute to disease, treatment, or delivery-related effects. In preclinical medicine, the design can also reduce the number of animals required for repeated measurements while preserving longitudinal information.
Modalities differ in the signal they capture. Bioluminescence and fluorescence can report signals from cells or molecular reporters, whereas magnetic resonance imaging and ultrasound visualize biological structures or processes through different imaging outputs. Selecting among them depends on the tissue, process, or reporter being followed and on maintaining consistent, defined conditions across serial measurements.
Temporal patterns show how a biological change unfolds rather than providing only a single endpoint. Relating those patterns to clinical or biological outcomes strengthens interpretation of preclinical studies. This is particularly relevant when disease progression, treatment response, or therapeutic delivery changes over multiple observation points, because the timing of an imaging signal can add context to its meaning.
Serial measurements should be acquired from the same living subject under defined experimental conditions. The resulting images or signals can then be organized by observation time and compared with later disease, treatment, or delivery-related outcomes. Keeping conditions defined is important because it helps distinguish temporal biological changes from differences introduced by the measurement setting.
Within medicine, this approach is useful when investigators need to characterize disease progression, monitor how a treatment performs over time, or assess therapeutic delivery. Repeated observations connect imaging findings with later clinical or biological outcomes, giving preclinical studies a temporal dimension that separate specimens measured at different times cannot provide.
Longitudinal In Vivo Imaging supports more precise evaluation of emerging therapies by showing how imaging signals change in relation to treatment and disease. It can reveal whether an intervention’s observed effects follow a meaningful temporal pattern and can support assessment of delivery alongside response. These measurements strengthen the preclinical evidence used to assess therapeutic strategies.