Orthotopic Implantation
Orthotopic Implantation - Related Videos
05:08
Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo
0 Views •
2023
This video demonstrates the protocol for implanting green fluorescent protein-expressing pancreatic cancer cells orthotopically into the pancreas of the mouse. The orthotopic mouse model helps in investigating the efficacy of novel therapeutics on primary and metastatic tumors.
03:49
Generating Renal Cell Carcinoma Model: A Protocol to Develop Orthotopic RCC Murine Model by Intrarenal Implantation of Cancer Cells
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2023
In this video, we develop an orthotopic mouse model of renal cell carcinoma. This model results in reproducible primary tumors in the injected kidney and metastatic tumors in the lung, allowing the evaluation of disease progression and treatment.
02:56
Orthotopic Injection: Implanting Tissue Specific Cancer Cells into an Adult Mouse
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2023
The video demonstrates the breast cancer orthotopic inoculation technique in a mouse model. This technique produces a high tumorigenesis rate with less variability in tumor size and shape compared to subcutaneous or non-surgical injection.
08:43
Orthotopic Mouse Model of Colorectal Cancer
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Cited by 109 •
2007
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
18:25
Orthotopic Liver Transplantation in Rats
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Cited by 31 •
2012
We present an easy-to-establish revision of the classical two-cuff technique for orthotopic liver transplantation in rat.
FAQs
The native anatomical environment can preserve spatial relationships and local signaling that influence how a graft interacts with surrounding tissue. These relationships may make the implanted organ, tissue, tumor, or engineered construct more physiologically representative than placement in an unrelated location. The resulting context helps investigators assess function and integration under conditions that more closely reflect the intended site.
Reconstruction may involve connections to blood vessels, ducts, or nerves, depending on the target tissue and the clinical or experimental protocol. These connections are not identical for every implantation, because each tissue has different anatomical requirements. Preserving or recreating the relevant pathways can support evaluation of graft function and the extent of integration within the host site.
Native-site placement maintains local anatomical and signaling conditions that can affect disease behavior and tissue interactions. In preclinical medicine, this context may improve physiological relevance when researchers study tumor progression, graft performance, or responses to treatment. It therefore provides information that may be less representative when a construct or tumor is evaluated outside its corresponding anatomical environment.
The procedure is tailored according to the tissue being implanted and the protocol governing its reconstruction. Researchers or clinicians determine which anatomical relationships and connections are relevant, including potential links to vessels, ducts, or nerves. Because requirements vary among organs, tissues, tumors, and engineered constructs, the operative approach cannot be treated as a single standardized reconstruction for every application.
Medicine uses this approach in organ transplantation, tissue reconstruction, and preclinical disease modeling. Its value is greatest when anatomical context affects the question being studied, such as whether a graft functions appropriately, integrates with surrounding tissue, or responds to treatment. The same principle can therefore support both therapeutic procedures and experimental systems designed to evaluate disease-related outcomes.
These models can support assessment of graft function, tumor progression, treatment response, and long-term integration. Because the implanted material occupies its corresponding anatomical environment, observations can incorporate relevant local relationships rather than focusing only on behavior in an unrelated site. In medicine, that added context helps connect structural placement with functional or disease-related outcomes over time.