Human Cortical Slices
Human Cortical Slices - Related Videos
02:58
Transplantation of Stem Cell-Derived Neuronal Progenitors into Human Cortical Slices
0 Views •
2025
Source: Palma-Tortosa, S., et.al. Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation. J. Vis. Exp. (2022)This video demonstrates the transplantation of stem cell-derived neuronal progenitor cells into human cortical tissue slices. The procedure involves isolating and resuspending progenitor cells in a basement membrane matrix, followed by their injection into a cortical slice placed on a transwell assembly. After incubation to allow...
04:59
Visualization of Amyloid-Beta Peptides in Human Cortical Brain Slices
0 Views •
2025
The video demonstrates a procedure for preparing human cortical brain slices from Alzheimer's disease patients for analyzing the deposition of amyloid-beta (Aβ) peptides using Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry (MALDI-IMS). The steps include preparing the slides, treating the tissue to enhance ionization, and analyzing the spatial distribution of amyloid-beta peptides.
07:13
Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
0 Views •
Cited by 3 •
2024
Here, we present the acute genetic manipulation of sliced human cortical organoids by electroporation. These cortical organoid models are particularly amenable to injection as ventricle-like structures can be readily identified after slicing, enabling the functional investigation of human cortical development, neurodevelopmental disorders, and cortical evolution.
03:10
Generation of Spontaneous and On-Demand Ictal Events in Mouse Brain Cortical Slices
0 Views •
2025
This video demonstrates the induction of ictal events in coronal cortical slices from a transgenic mouse expressing light-activated cation channels in cortical excitatory pyramidal neurons. The slice is placed in a recording chamber, and a recording electrode is inserted into the superficial cortical layer. Upon perfusion with a seizure-inducing drug that blocks potassium channels, the neurons become hyperexcitable and generate spontaneous ictal events. To generate on-demand ictal events, a...
02:59
Time-Lapse Imaging of Cortical Neuron Radial Migration in Transduced Mouse Embryonic Brain Slices
0 Views •
2025
Source: Wiegreffe, C., et. al., Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation. J. Vis. Exp. (2017) This video demonstrates time-lapse confocal microscopy to observe cortical neuron radial migration from the subventricular zone to the cortical plate via radial glial scaffolds in transduced mouse embryonic brain slices.
FAQs
Preserving local neurons, glial cells, synaptic connections, and aspects of cortical circuitry is what makes these preparations experimentally informative. Because several cellular elements remain together, investigators can examine interactions within a local cortical network rather than observing neurons in isolation. This arrangement supports direct analysis of how synaptic transmission and network excitability emerge from connected tissue.
The oxygenated physiological medium provides the controlled environment in which living tissue is maintained outside the body. Oxygenation and physiological conditions help preserve the cellular and synaptic components needed for observation. By keeping the preparation in this medium, researchers can study activity and responses while varying experimental conditions in a controlled setting.
Human Cortical Slices complement both isolated-cell preparations and experiments in intact brains by occupying an intermediate scale. They retain local neurons, glial cells, synaptic connections, and circuitry that isolated cells lack, yet they allow more direct and controlled observation than studies performed in a complete brain. This bridge helps connect cellular mechanisms with circuit-level behavior.
The workflow begins with surgically removed cortical tissue, which is prepared as thin sections and maintained in oxygenated physiological medium. Once preserved outside the body, researchers can record electrical activity or observe cellular responses under controlled conditions. This sequence links tissue preparation with measurements of circuit function and cellular behavior.
Electrical activity and cellular responses are the principal observable outcomes available from this preparation. Electrical recordings can address synaptic transmission and network excitability, while cellular observations reveal how preserved neural elements respond under experimental conditions. Together, these readouts let investigators connect changes at the cellular level with effects on local cortical circuitry.
Human Cortical Slices are especially useful when researchers need to examine disease-related cellular effects or responses to therapeutic compounds in human cortical tissue. The preparation permits these questions to be studied while local neurons, glial cells, synaptic connections, and circuitry remain together. Findings can therefore relate compound or disease effects to both cells and networks.
The preserved local circuitry allows investigators to examine sensory and cognitive circuit properties in human cortical tissue while working under controlled conditions. This is valuable because the preparation supports direct electrical recording and cellular observation within connected tissue, rather than limiting analysis to isolated cells. It can therefore reveal how circuit-level activity relates to the responses of individual neural elements.