Recruitment Curve
Recruitment Curve - Related Videos
05:52
An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
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2025
The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.
04:28
Lung Tumor Cell Recruitment Assay
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2019
This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis.
11:30
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
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Cited by 28 •
2015
The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.
07:43
Calibration Curves
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Calibration curves are used to understand the instrumental response to an analyte and predict the concentration in an unknown sample. Generally, a set of standard samples are made at various concentrations with a range than includes the unknown of interest and the instrumental response at each concentration is recorded. For more accuracy and to understand the error, the response at each concentration can be repeated so an error...
04:29
Monitoring Neutrophil Recruitment Dynamics and Bacterial Burden at the Infection Site in a Murine Model
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2025
This video demonstrates in vivo imaging to study the neutrophil recruitment dynamics at an infection site in an immunocompromised, transgenic mouse with GFP-expressing neutrophils. It involves injecting luminescent bacteria to cause an infection. This leads to neutrophil recruitment to the infection site, whose tracking is done by imaging.
FAQs
Weak stimulation reaches the most excitable neurons or neural elements first. As intensity rises, less excitable axons, motor units, or circuit elements also become engaged, expanding the measured response. This ordered increase helps reveal how heterogeneous neural populations respond to input, while the eventual plateau indicates that further stimulation no longer produces substantial additional recruitment.
Threshold indicates the stimulation level at which measurable recruitment begins, whereas slope describes how rapidly additional neural elements become engaged as intensity increases. The maximum response reflects the greatest response reached under the tested conditions. Considering these features together provides more information than any single value and helps characterize differences in neural excitability.
Changes in the curve can indicate that neural elements are being recruited differently, but the measured response may also be influenced by synaptic or muscular responsiveness. Examining threshold, slope, and maximum response together helps researchers separate these possibilities conceptually. This distinction is important when interpreting whether an observed change primarily reflects neural recruitment or downstream response properties.
Researchers apply stimuli across a range of intensities and record the resulting neural response, either as response size or as the probability of observing a response. They then relate each response measure to its corresponding stimulus intensity. The resulting pattern can be examined for its threshold, rate of increase, and plateau, providing complementary measures of recruitment behavior.
Recruitment curves support comparisons between experimental conditions when researchers want to quantify changes in neuronal or corticospinal excitability. They can be used to examine effects associated with learning, injury, disease, or neuromodulation. Comparing curve features across conditions may show whether recruitment begins at a different intensity, progresses differently, or reaches a different maximum response.
Depending on the experiment, the measured recruitment pattern may reflect the engagement of axons, motor units, or broader circuit elements. In neuroscience, the approach can also quantify corticospinal excitability, linking stimulation intensity to responses within that pathway. This flexibility allows the same analytical framework to describe recruitment at different organizational levels of the nervous system.