Robotic Liquid Handler
Robotic Liquid Handler - Related Videos
08:00
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
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Cited by 23 •
2015
The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
10:47
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
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Cited by 13 •
2013
TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.
09:58
Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing
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Cited by 37 •
2015
A protocol for robotic printing of cancer cell spheroids in a high throughput 96-well plate format using an aqueous two-phase system is presented.
10:12
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
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Cited by 21 •
2016
A high-throughput, automated, tobacco protoplast production and transformation methodology is described. The robotic system enables massively parallel gene expression and discovery in the model BY-2 system that should be translatable to non-model crops.
08:24
Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
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Cited by 5 •
2008
The Ugi reaction has proved to be a convenient way to quickly create diverse libraries of compounds. It involves the reaction of an amine, an aldehyde, a carboxylic acid and an isonitrile typically in methanol at room temperature. In this video, we utilize a 48-slot Mettler-Toledo MiniBlock equipped with filtration tubes and a Mettler-Toledo MiniMapper automated liquid handler was used to deliver the reagents and solvent. The parameters of interest were the concentration, the solvent...
FAQs
The programmed sequence determines when the pipetting head moves between labware, aspirates, dispenses, and mixes liquids. Coordinating these actions makes each sample encounter the intended reagents in the intended order, which supports consistent preparation across repeated conditions. This controlled timing is especially useful when neuroscience experiments require many samples or standardized assay setups.
Tip selection is an important part of the transfer strategy. Robotic liquid handlers can use disposable or fixed tips while moving defined volumes among tubes, microplates, and other labware. This flexibility allows protocols to match the materials and workflow being used, helping maintain consistent liquid transfers during reagent distribution, cell-based assay preparation, or nucleic acid workflows.
Reproducibility depends on coordinating defined volumes, pipetting sequence, movement between labware, and mixing steps within the protocol. These variables determine how consistently samples and reagents are prepared from one run to the next. Standardizing them reduces variation associated with manual pipetting and supports more comparable measurements across repeated experimental conditions.
Automation reduces the amount of manual pipetting required while applying the same programmed handling sequence across many samples. This combination can improve consistency, reduce handling errors, and increase throughput compared with workflows performed manually. The advantage becomes more important when experiments involve repeated conditions, large sample sets, or screening designs that must be prepared in a standardized way.
A workflow begins with a programmed protocol that specifies the liquid-handling sequence and defined transfer volumes. The pipetting head then moves between the selected tubes, microplates, or other labware, aspirates and dispenses liquids, and performs programmed mixing when required. This sequence can be applied repeatedly to support consistent sample preparation across an experiment.
In neuroscience, these instruments support reagent distribution, preparation of cell-based assays, nucleic acid workflows, and screening experiments. Their value lies in applying standardized handling across many samples and conditions rather than in a single specialized assay. This makes them useful for studies that need scalable preparation and comparable measurements across repeated experimental setups.
Automated handling can produce more standardized sample preparation, improved measurement consistency, and greater experimental throughput. These outcomes are particularly relevant to screening experiments and workflows with many repeated conditions, where manual variation can complicate comparisons. By controlling the handling sequence and transfer volumes, the system helps make larger neuroscience studies more manageable and reproducible.