S35 Methionine Incorporation
S35 Methionine Incorporation - Related Videos
08:09
Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery
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Cited by 1 •
2019
This work presents the preparation of methionine functionalized biocompatible block copolymers (mBG) via the reversible addition-fragmentation chain transfer (RAFT) method. The plasmid DNA complexing ability of the obtained mBG and their transfection efficiency were also investigated. The RAFT method is very beneficial for polymerizing monomers containing special functional groups.
08:24
Bioinspired Soft Robot with Incorporated Microelectrodes
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Cited by 9 •
2020
A bioinspired scaffold is fabricated by a soft photolithography technique using mechanically robust and electrically conductive hydrogels. The micropatterned hydrogels provide directional cardiomyocyte cell alignment, resulting in a tailored direction of actuation. Flexible microelectrodes are also integrated into the scaffold to bring electrical controllability for a self-actuating cardiac tissue.
13:36
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
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Cited by 1 •
2015
Here, we present a protocol to generate a proof-of-principle divalent adenovirus type 5 (Ad5) vector Ad5/H5-HVR1-KWAS-HVR5-His6 by utilizing the Antigen Capsid-Incorporation strategy. This vector was demonstrated to exhibit qualitative fitness, the capability to escape Ad5-positive sera in vitro, and the antigenicity as well as immunogenicity to the incorporated antigens.
07:39
Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
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Cited by 3 •
2022
This protocol describes how to perform cell-type-specific protein labeling with azidonorleucine (ANL) using a mouse line expressing a mutant L274G-Methionine tRNA synthetase (MetRS*) and the necessary steps for labeled cell-type-specific proteins isolation. We outline two possible ANL administration routes in live mice by (1) drinking water and (2) intraperitoneal injections.
06:21
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
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Cited by 4 •
2018
This protocol presents a novel in vitro bead assay that more appropriately models the process of in vivo sprouting angiogenesis by incorporating pericytes. This modification enables the bead assay to more faithfully recapitulate the heterotypic cellular interactions between endothelial cells and mural cells that are critical for angiogenesis.
FAQs
During translation, cells draw on the available methionine pool as they assemble polypeptides. When sulfur-35-labeled methionine is used, newly formed proteins acquire the radioactive label. Measuring the resulting signal therefore provides a quantitative readout of protein production occurring during the labeling period, rather than simply indicating that proteins are present.
The radioactive signal reflects methionine incorporated while new polypeptides are being produced. Consequently, the measurement links an experimental condition to protein synthesis during a defined period. Comparing signal among conditions can reveal whether neuronal activity, development, or a treatment is associated with increased or decreased translational output.
Translation converts the information carried by cellular messenger molecules into polypeptides, and it is the stage at which labeled methionine becomes part of newly synthesized proteins. Measuring this incorporation gives researchers a molecular indicator of translational activity in neurons or neural tissues, helping connect cellular responses with changes in protein production.
Neuronal adaptation can involve altered production of proteins that support changing cellular states. By tracking newly synthesized proteins, this method allows protein production to be examined after neuronal activity, during synaptic plasticity, across development, or following an experimental treatment. The measured radioactive signal supplies an outcome for comparing translational responses across those contexts.
A typical workflow begins by providing cells or neural tissue with sulfur-35-labeled methionine during protein synthesis. The label is incorporated into cellular polypeptides as translation proceeds. Researchers then detect and quantify the radioactive signal associated with the newly produced proteins, using the measurement to compare protein synthesis between experimental conditions.
This approach is useful when the central question concerns newly produced proteins rather than protein presence alone. In neuroscience, it can assess translational changes in neurons and neural tissues associated with neuronal activity, synaptic plasticity, development, or experimental treatments. Its value lies in relating those conditions to measurable changes in protein production.