Stereotactic Manipulation
Stereotactic Manipulation - Related Videos
04:15
Stereotactic Microinjection of a Viral Vector for Gene Manipulation in a Mouse Pup Striatum
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2025
Source: Chen, S., et al., Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains. J. Vis. Exp. (2018)This video demonstrates the stereotaxic injection of a viral vector encoding Cre recombinase and green fluorescence protein or GFP into the striatum of a genetically modified mouse pup to achieve gene manipulation by excising a loxP-flanked STOP cassette, activating red fluorescence protein or RFP as a reporter.
10:35
Stereotactic Radiosurgery for Gynecologic Cancer
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Cited by 14 •
2012
Stereotactic body radiotherapy (SBRT) involves image-guided, ablative radiation delivered to cancer targets refractory to chemotherapy or to conventional radiation treatment. The robotic-armed Cyberknife SBRT system, using sophisticated target localization, delivers hypofractionated radiation doses capable of sterilizing cancer targets. This article will consider new therapeutic roles of SBRT for gynecological cancers.
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 50 •
2014
Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
07:54
Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors
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Cited by 15 •
2015
Stereotactic injection of lentiviruses expressing cDNAs or shRNAs can modulate gene expression in specific brain areas of mice. Here, we present a protocol to combine stereotactic injections with behavioral tasks, such as the Open Field Test (OFT) and the Forced Swim Test (FST).
05:08
A Stereotactically Guided Ablation Technique for Auditory Cortex Removal in Rats
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2025
This video demonstrates a stereotactically-guided surgical procedure for ablating the auditory cortex in a rat. The steps include identifying anatomical landmarks in an anesthetized rat secured in a stereotactic frame, creating a window on the skull above the auditory cortex, aspirating the cortical layers, and providing post-operative care to assess the impact of the ablation on sound processing.
FAQs
Landmarks such as bregma provide fixed anatomical reference points for assigning three-dimensional coordinates. Researchers relate the intended brain region to these landmarks, then use the resulting coordinates to guide an instrument toward a small, anatomically defined target. This reference-based approach helps standardize placement across experiments and improves the reproducibility of targeted interventions.
A stereotaxic frame or comparable apparatus stabilizes the subject’s head during instrument placement. This limits changes in the head’s position relative to the coordinate system, allowing the selected coordinates to remain aligned with the intended brain region. Stable positioning is therefore central to accurate targeting and to consistent comparisons among experimental subjects.
The approach can guide electrodes, guide cannulas, injection needles, and other instruments into selected brain regions. The instrument determines the type of intervention or measurement that follows: electrodes can record or stimulate neural activity, whereas cannulas and needles can support drug delivery. Thus, the same positioning framework can serve different experimental purposes.
A typical workflow begins by stabilizing the subject’s head in the stereotaxic apparatus, identifying an anatomical reference such as bregma, and using coordinates to select the intended target. The researcher then positions the chosen electrode, cannula, needle, or other tool at that location. This sequence links physical placement to the planned neural intervention or measurement.
Researchers choose this approach when an experiment requires access to a small, anatomically defined brain region. Its spatial precision supports localized drug delivery, neural recording or stimulation, and creation of lesions. By restricting the intervention or measurement to a selected structure, investigators can more directly examine relationships between that region and behavior, physiology, or disease-related processes.
The resulting experiments can connect activity or manipulation within a specific neural structure to observable outcomes. Depending on the instrument and intervention, researchers may examine neural activity, stimulate a circuit, deliver a drug, or create a localized lesion, then relate those changes to behavior, physiology, or disease-related processes in animal models.