Helium Leak Detection

Helium leak detection is a sensitive method for finding and measuring tiny openings in sealed or vacuum systems by using helium as a tracer gas. In a typical test, the component is evacuated while helium is applied outside or introduced inside; any gas entering through a leak reaches a mass spectrometer, which ionizes helium and identifies it by its characteristic mass-to-charge ratio. This principle supports quantitative leak-rate measurements and helps distinguish true leaks from background signals. It is used in vacuum chambers, cryogenic equipment, semiconductor manufacturing, space hardware, and other systems where contamination prevention, pressure stability, and reliable performance matter.

Helium Leak Detection - Related Videos

Research

JoVE Journal - Medicine

An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

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Cited by 68 •

2018

In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues.

Intrathecal Application of a Fluorescent Dye for the Identification of Cerebrospinal Fluid Leaks in Cochlear Malformation

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Cited by 2 •

2020

Intrathecally applied fluorescein is used to achieve intraoperative visualization of CSF leaks. This protocol describes a lumbar puncture, the application of 5% fluorescein, and intraoperative visualization using a fully digital microscope.

Research

JoVE Journal - Biology
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Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

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Cited by 17 •

2011

The endoplasmic reticulum plays a key role in protein biogenesis and in calcium homeostasis. We have established an experimental system that allows us to address the role of Ca2+ leak channels and to characterize their putative regulatory mechanisms. This system involves siRNA mediated gene silencing and live cell Ca2+ imaging.

Education

JoVE Science Education - Chemistry

Gas Chromatography (GC) with Flame-Ionization Detection

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2023

Source: Laboratory of Dr. B. Jill Venton - University of Virginia Gas chromatography (GC) is used to separate and detect small molecular weight compounds in the gas phase. The sample is either a gas or a liquid that is vaporized in the injection port. Typically, the compounds analyzed are less than 1,000 Da, because it is difficult to vaporize larger compounds. GC is popular for environmental monitoring and industrial applications because it is very reliable and can be run nearly continuously.

Detecting Reactive Oxygen Species

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2023

Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...

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