In Situ Analysis
In Situ Analysis - Related Videos
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
14:53
In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
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Cited by 3 •
2018
We describe a procedure for the detection of chemical elements present in situ in human cells as well as their in vitro quantification. The method is well-suited to any cell type and is particularly useful for quantitative chemical analyses in single cells following in vitro metal oxide nanoparticles exposure.
09:44
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
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Cited by 20 •
2011
We present a protocol for structural and compositional analysis of natural oral biofilm from orthodontic appliances with in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). Oral biofilm samples were collected from palatal expanders, scraping acrylic-resin flakes off their surface and referring them for molecular processing.
06:01
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
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Cited by 4 •
2016
Here we present a protocol to detect miRNA expression in breast cancer patient samples using miRNA in situ hybridization.
09:31
Preparation of Authigenic Pyrite from Methane-bearing Sediments for In Situ Sulfur Isotope Analysis Using SIMS
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Cited by 2 •
2017
Analyses of the sulfur isotopic composition (δ34S) of pyrite from methane-bearing sediments have typically focused on bulk samples. Here, we applied secondary ion mass spectroscopy to analyze the δ34S values of various pyrite generations to understand the diagenetic history of pyritization.
FAQs
Controlled variables such as temperature, pressure, and electric or magnetic fields provide a defined environment for observing change. Researchers can relate a measured response to a specific adjustment rather than to an uncontrolled shift in conditions. This approach helps connect physical behavior with its driving factors, particularly when studying phase transitions, transport phenomena, or field-dependent responses.
Removing a material or system from its operating environment can change the structure or behavior that researchers want to understand. Measurements collected during operation preserve the relationship between conditions and response, including changes that may not remain afterward. This is especially important for dynamic interactions, mechanical responses, and transformations whose evidence can be altered by handling or cooling.
Real-time measurement links the timing of an applied condition with the resulting structural or behavioral change. A probe, detector, imaging system, or spectroscopic measurement can follow how the system evolves rather than only recording an initial and final state. That sequence helps distinguish ongoing processes and supports interpretation of mechanisms behind phase changes, transport, and mechanical behavior.
These measurement tools record different forms of evidence while the material or system remains under investigation. Imaging can follow visible or spatial changes, detectors can register responses, and spectroscopy can track changes associated with the system’s state. Used with controlled temperature, pressure, electric, or magnetic conditions, they provide complementary information about structure and behavior.
A typical workflow establishes the material, system, or process in its operating setting, applies or controls relevant conditions, and records responses as those conditions change. Researchers then relate the measurements to structural or behavioral evolution. The specific combination of probes, detectors, imaging, or spectroscopy depends on whether the goal is to examine transitions, mechanical response, transport, or dynamic interactions.
The approach is used in condensed matter physics, materials science, nanotechnology, and device development. It is valuable when researchers need to connect a material’s structure with behavior during operation rather than after processing. Applications include studying phase transitions, mechanical responses, transport phenomena, and dynamic interactions, providing information that can guide understanding of materials and physical devices.