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Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
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JoVE Journal Biologie
Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis

Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis

DOI:
10.3791/1736-v

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10:28 min

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February 08, 2010

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, , , ,
1Department of Molecular, Cellular, and Developmental Biology,University of Michigan-Ann Arbor

Kapitel

  • 00:00Titel
  • 00:45Introduction
  • 01:18Growing Cultures of Cells for Microscopy
  • 04:55Prepare the Cells for Imaging
  • 07:58Visualization of the Cells
  • 09:11Representative Images of DNA Damage Response in Bacillus subtills
  • 10:05Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A detailed protocol is described for imaging the real time formation of DNA repair complexes in Bacillus subtilis cells.

Tags

Mismatch RepairCellular ResponsesDNA DamageBacillus SubtilisGene ExpressionProtein RedistributionProtein ComplexesDNA LesionsDNA Base PairsFluorescence MicroscopyVisualizing Responses To DNA LesionsQuantifying Responses To DNA LesionsDNA Replication StatusSubcellular ArchitectureLiving CellFluorescent Reporter ProteinsDNA Repair MachineryBacterial CellsGenotoxic Stress ResponsesFluorescence Microscopy ProtocolImaging Mismatch Repair ProteinsHomologous RecombinationDNA ReplicationSOS-inducible Protein

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