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JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets (Video) | JoVE

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Journal (Biochemistry)

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Journal (Biochemistry)

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets (Video) | JoVE

Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

Article DOI: 10.3791/200052-v • 01:18 min • June 2nd, 2023

June 2nd, 2023 •

Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra, Coimbra Health School

Tags

Fluorescent-stained Lipid Droplets 3D Reconstruction Hepatic Steatosis Assessment Histological Staining Microvesicular Steatosis Oil Red Pro-BODIPY Hydrophobicity Lipid Droplet Tagging Environment Polarity Immunochemistry Techniques Subcellular Antigens Lipid Droplet Biogenesis Lipid Droplet Dynamics Microvesicular Hepatic Steatosis

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