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Generation of Rat Intestinal 2D Monolayers from 3D Organoids (Video) | JoVE

Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

Article DOI: 10.3791/200274-v • 02:47 min • June 23rd, 2023
June 23rd, 2023 •
*1, *1,2, 3,4, 1,5,6
1Department of Genetics, Yale School of Medicine, 2Department of Pediatrics, Yale School of Medicine, 3Department of Pediatrics/Gastroenterology and Hepatology, Yale School of Medicine, 4Department of Cellular and Molecular Physiology, Yale School of Medicine, 5Yale Stem Cell Center, Yale School of Medicine, 6Yale Cancer Center, Yale School of Medicine
* These authors contributed equally

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Rat Intestinal Organoids Genetic Manipulation Lentiviral Infection Transient Transfection Organoid Model Stem Cell Behavior Cell Fate Physiology Mouse Intestinal Organoids Human Intestinal Organoids Physiological Relevance Genetic Tools Pharmacological Treatments Higher Throughput Studies
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