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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies (Video) | JoVE

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Journal (Immunology and Infection)

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Journal (Immunology and Infection)

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies (Video) | JoVE

PBMC Collection and Cryostorage for Mitochondrial Bioenergetics Analysis

Article DOI: 10.3791/200888-v • 02:46 min • October 20th, 2023

October 20th, 2023 •

Fabian Dieter¹, Jendrik Grube¹, Tim Birkenhauer¹, Alice Quentin¹, Gunter P. Eckert¹

¹Biomedical Research Center, Institute of Nutritional Sciences, Justus-Liebig-University of Giessen

Tags

Cryopreservation Bioenergetic Evaluation Peripheral Blood Mononuclear Cells Mitochondria Mitochondrial Dysfunction Metabolic Diseases Aging Oxidative Phosphorylation Energetic Homeostasis PBMCs Mitochondrial Function Disease Conditions Human Samples Limitations Cryopreserved Samples Freshly Prepared Cells Isolating PBMCs Cryopreserving PBMCs Bioenergetic Function Cell Number And Viability Adenosine Triphosphate Levels Respiratory Chain Activity Human Blood Samples Clinical Studies Multicentral

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