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Journal / Genetics / A Guide to Precise Gene Disruption in Primary… / Preparation for Electroporation and…
JoVE Journal
Genetics

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JoVE Journal Genetics
Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
Journal (Genetics)
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
Journal (Genetics)
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
 

Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages

A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages

Article DOI: 10.3791/200896-v • 04:03 min • August 4th, 2023
August 4th, 2023 •
1, 1, 1,2
1Department of Internal Medicine, University of California, Davis, 2Department of Medical Microbiology and Immunology, University of California, Davis

Tags

Electroporation Cas9-sgRNA Complexes CRISPR/Cas9 Genome Editing Frameshift Mutations Small Insertions And Deletions (indels) Gene Function Single-guide RNAs (sgRNA) Ribonucleoprotein Complexes (RNPs) Editing Efficiency Sanger Sequencing Analysis Program
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