Encyclopedia of Experiments: Biological Techniques
Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Transcript
Begin by adding 1.5 micromolar of freshly-prepared fibrinogen in 200 microliters of clot formation buffer to each Aβ negative 42-containing and control wells, and incubate the plate on a rotating platform at room temperature. After 30 minutes, simultaneously add 30 microliters of freshly-prepared thrombin solution directly into the center of each fibrinogen-containing well to initiate clot formation, and immediately read the absorbance of the in-vitro clots at 350 nanometers, repeating the measurement every 30 to 60 seconds over the course of 10 minutes.