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Journal / Immunology and Infection / 高效转染树突状细胞的无细胞的成熟优化的协议…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
高效转染树突状细胞的无细胞的成熟优化的协议
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高效转染树突状细胞的无细胞的成熟优化的协议

Article DOI: 10.3791/2766-v • 08:08 min • July 8th, 2011
July 8th, 2011 •
*1, *1, 1, 1
1Center for Translational Systems Biology and Department of Neurology, Mount Sinai School of Medicine
* These authors contributed equally

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Summary
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我们目前我们优化的高通量nucleofection协议，要么质粒DNA或不会造成细胞的成熟的siRNA转染初级人类单核细胞衍生的树突状细胞的有效方法。我们进一步提供证据证明为成功的siRNA沉默靶基因的mRNA和蛋白水平的RIG - I。

Tags

免疫学杂志，53期，树突状细胞，nucleofection，高通量，RNA干扰，干扰素信号
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