JoVE Journal
Bioengineering
Bioengineering
Dieser Inhalt ist Open Access.
Kapitel
Summary
Laser axotomy followed by time-lapse imaging is a sensitive way to assay the effects of mutations in C. elegans on axon regeneration. A high quality, but inexpensive, laser ablation system can be easily added to most microscopes. Time lapse imaging over 15 hours requires careful immobilization of the worm.