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Bioengineering

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JoVE Journal Bioengineering
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
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A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Article DOI: 10.3791/50597-v • 12:21 min • August 6th, 2013
August 6th, 2013 •
1, 2, 2,3, 4, 2
1Department of Systems Biology, Sandia National Laboratories, 2Department of Biotechnology and Bioengineering, Sandia National Laboratories, 3Canon U.S. Life Sciences, 4Department of Advanced Systems Engineering and Deployment, Sandia National Laboratories

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Summary
Automatic Translation

We have developed an automated cell culture and interrogation platform for micro-scale cell stimulation experiments. The platform offers simple, versatile, and precise control in cultivating and stimulating small populations of cells, and recovering lysates for molecular analyses. The platform is well suited to studies that use precious cells and/or reagents.

Tags

Automated Platform Micro-scale Cell Stimulation Experiments In Vitro Analysis Conventional Methods Equipment Culture Size Reduction Microfluidics-based Automated Systems Technology Platform Fibronectin-coated Microcapillaries Cell Perfusion Chambers Digital Microfluidics (DMF) Device Transfer Microcapillaries High-precision Syringe Pump
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