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Journal / Immunology and Infection / Microglia cultura do Sistema Nervoso Central…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Microglia cultura do Sistema Nervoso Central Neonatal e Adulto
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Microglia cultura do Sistema Nervoso Central Neonatal e Adulto

Article DOI: 10.3791/50647-v • 11:28 min • August 9th, 2013
August 9th, 2013 •
*1,2, *2,3, *2,3, 2
1Program in Neuroscience, Stony Brook University, 2Department of Pharmacological Sciences, Stony Brook University, 3Program in Molecular and Cellular Pharmacology, Stony Brook University
* These authors contributed equally

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Nós delinear métodos para a rápida e eficiente isolamento / cultura da microglia viável a partir do córtex cerebral neonatal e adulto medula espinhal. A dissecção e revestimento de microglia cortical pode ser realizado dentro de 90 minutos, com a colheita da microglia subseqüente ocorrendo ~ 10 dias após a dissecção inicial.

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Imunologia Edição 78 Neurociência Neurobiologia Biologia Celular Biologia Molecular Medicina Engenharia Biomédica Bioengenharia Anatomia Fisiologia imunossupressão ciências da vida biologia animal modelos animais bioquímica microglia córtex mouse neonatal cultura celular espinal medula adulto da cultura de tecidos modelo animal
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