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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Article DOI: 10.3791/50772-v • 14:43 min • September 23rd, 2013
September 23rd, 2013 •
1, 1, 1, 1, 1, 1, 1, 1
1Center for Systems Biology, Massachusetts General Hospital

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Summary
Automatic Translation

We present a method for rapid, reversible immobilization of small molecules and functionalized nanoparticle assemblies for Surface Plasmon Resonance (SPR) studies, using sequential on-chip bioorthogonal cycloaddition chemistry and antibody-antigen capture.

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Microfluidic On-chip Capture-cycloaddition Reaction Reversible Immobilization Small Molecules Multi-component Structures Biosensor Applications Covalent Bioorthogonal [4+2] Cycloaddition Reaction Trans-cyclooctene (TCO) 1,2,4,5-tetrazine (Tz) Microfluidic Immobilization TCO/Tz-derivatized Molecules Real-time Monitoring Continuous Flow Conditions Surface Plasmon Resonance (SPR) Non-covalent Antigen-antibody Capture Component On-chip Assembly Interaction Studies Multi-component Structures Immobilization Experiments Biosensor Chip AP1497 FK506-binding Protein 12 (FKBP12) Nanoparticle
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