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Immunology and Infection

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Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Article DOI: 10.3791/51678-v • 09:04 min • July 9th, 2014
July 9th, 2014 •
1, 1, 2
1Department of Medicine, Roswell Park Cancer Institute, 2Department of Medicine, School of Medicine and Biomedical Sciences, University of Buffalo

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Summary
Automatic Translation

A method to evaluate the ability of isolated mouse alveolar macrophages to control the growth of phagocytosed Aspergillus spores by confocal microscopy.

Tags

Anti-fungal Activity Isolated Alveolar Macrophages Confocal Microscopy Lung Interface Host Cells Microbes Microbial Products Phagocytic Cells Inhaled Fungi Aspergillus Motifs Pathogen Recognition Receptors Inflammatory Responses NADPH Oxidase Reactive Oxygen Intermediates (ROIs) Host Defense Macrophage-mediated Host Defense A. Fumigatus Mouse Alveolar Macrophages (AMs) Control Of Phagocytosed Aspergillus Spores (conidia) Bronchoalveolar Lavage (BAL) Glass Coverslips Green Fluorescent Protein (GFP) Confocal Microscopy Assessment
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