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Journal / Biology / 基于海拉细胞自由表达系统中的表达
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基于海拉细胞自由表达系统中的表达
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基于海拉细胞自由表达系统中的表达

Article DOI: 10.3791/52772-v • 09:03 min • June 10th, 2015
June 10th, 2015 •
1, 1
1Department of Biological, Geological, and Environmental Sciences, Cleveland State University

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基于细胞系统疟疾蛋白的表达仍然具有挑战性。我们证明两步并一步IVT（ 体外翻译）无细胞表达系统从HeLa细胞表达的重组疟疾蛋白质棒状体。我们使用的Ni树脂基于亲和性的纯化系统纯化棒状体蛋白质。

Tags

生物化学，100期，无细胞体外转录翻译，HeLa细胞自由表达，蛋白质棒状体，哺乳动物细胞表达系统，
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