LC-MS-Analyse von menschlichen Blutplättchen als Plattform für ein Studium Mitochondrial Metabolism
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April 04, 2016
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Hier zeigen wir isolierten menschlichen Blutplättchen kann als zugänglich ex vivo - Modell verwendet werden metabolische Anpassungen in Antwort auf den Komplex I Rotenon Inhibitor zu studieren. Dieser Ansatz beschäftigt Isotopenverfolgung und relative Quantifizierung durch Flüssigchromatographie-Massenspektrometrie und kann auf eine Vielzahl von Studiendesigns angewendet werden.
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Cite this Article
Worth, A. J., Marchione, D. M., Parry, R. C., Wang, Q., Gillespie, K. P., Saillant, N. N., Sims, C., Mesaros, C., Snyder, N. W., Blair, I. A. LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism. J. Vis. Exp. (110), e53941, doi:10.3791/53941 (2016).
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