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Journal / Developmental Biology / Metoder til at studere Mrp4-holdige…
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Developmental Biology

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JoVE Journal Developmental Biology
Metoder til at studere Mrp4-holdige Makromolekylær Komplekser i forordningen af ​​fibroblast Migration
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Metoder til at studere Mrp4-holdige Makromolekylær Komplekser i forordningen af ​​fibroblast Migration

Article DOI: 10.3791/53973-v • 10:43 min • May 19th, 2016
May 19th, 2016 •
*1,2, *1, 1,2
1Division of Pulmonary Medicine, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center, 2Department of Physiology, University of Tennessee Health Science Center
* These authors contributed equally

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Summary
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MRP4 regulerer forskellige cykliske nukleotid-afhængig signalering arrangementer, herunder en nylig belyst rolle i celle migration. Vi beskriver en direkte, men flerstrenget tilgang at optrævle de nedstrøms molekylære mål for MRP4 resulterer i identifikation af et unikt MRP4 interactome der spiller centrale roller i finjusteret regulering af fibroblast migration.

Tags

Developmental Biology multiresistens protein 4 (MRP4) Actin cAMP-afhængig protein kinase (PKA) Migration Ingenuity Pathway Analysis (IPA) High-Content Mikroskopi Förster resonans (FRET).
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