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Journal / Biochemistry / Método para a identificação de molécula pequena…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Método para a identificação de molécula pequena inibidores da interacção proteína-proteína entre HCN1 e TRIP8b
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Journal (Biochemistry)
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
 
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Método para a identificação de molécula pequena inibidores da interacção proteína-proteína entre HCN1 e TRIP8b

Article DOI: 10.3791/54540-v • 10:20 min • November 11th, 2016
November 11th, 2016 •
*1, *1, 2, 3, 1, 1, 4, 1,5
1Davee Department of Neurology and Clinical Neurosciences, Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery, Northwestern University, 3Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences, Northwestern University, 5Department of Physiology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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A interacção entre os canais HCN e a sua sub-unidade auxiliar tem sido identificada como um alvo terapêutico em Perturbação Depressiva Major. Aqui, um método baseado em polarização de fluorescência para identificar pequenas moléculas inibidoras desta interacção proteína-proteína, é apresentada.

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Bioquímica Edição 117 high-throughput screening descoberta de medicamentos interações proteína-proteína a polarização de fluorescência HCN TRIP8b
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