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Journal / Biochemistry / LERLIC-MS / MS for inngående karakterisering og…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
LERLIC-MS / MS for inngående karakterisering og kvantifisering av glutamin og asparagin Deamidering i hagle Proteomics
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LERLIC-MS / MS for inngående karakterisering og kvantifisering av glutamin og asparagin Deamidering i hagle Proteomics

Article DOI: 10.3791/55626-v • 08:01 min • April 9th, 2017
April 9th, 2017 •
*1, *1, 1
1Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University
* These authors contributed equally

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Her presenterer vi en trinn-for-trinn-protokollen for lang lengde elektrostatiske avstøtning-hydrofil interaksjonskromatografi-tandem massespektrometri (LERLIC-MS / MS) -metoden. Dette er en roman metode som gjør det mulig for første gang kvantifisering og karakterisering av glutamin og asparagin deamideringsseter isoformer av hagle proteomikk.

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Biochemistry utgave 122 deamidering glutamin asparagin ERLIC LERLIC degenerative proteinmodifiseringer DPMS Posttranslasjonelle modifikasjoner PTMs transamidering Long-lengde anionbytter-LAX Shotgun proteomikk HILIC.
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