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Journal / Biochemistry / Påvisning og visualisering af…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Påvisning og visualisering af DNA-skader-inducerede proteinkomplekser i suspensionscellekulturer ved anvendelse af Proximity Ligation Assay
Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
Journal (Biochemistry)
Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
Journal (Biochemistry)
Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
 
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Påvisning og visualisering af DNA-skader-inducerede proteinkomplekser i suspensionscellekulturer ved anvendelse af Proximity Ligation Assay

Article DOI: 10.3791/55703-v • 13:10 min • June 9th, 2017
June 9th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Pathology, Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

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Her er det demonstreret, hvordan in situ Proximity Ligation Assay (PLA) kan anvendes til at detektere og visualisere de direkte protein-proteininteraktioner mellem ATM og p53 i suspensionscellekulturer udsat for genotoksisk stress.

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Biokemi udgave 124 DNA-beskadigelse DNA-reparation ATM p53 suspensionscellekultur BCR-ABL + B-celle akut lymfoblastisk leukæmi protein-proteininteraktion,
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