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Cancer Research

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JoVE Journal Cancer Research

Evaluering af celle Invasion og migrationsprocessen

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

Journal (Cancer Research)

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

Evaluating In Vitro DNA Damage Using Comet Assay

Journal (Cancer Research)

Evaluating In Vitro DNA Damage Using Comet Assay

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Evaluering af celle Invasion og migrationsprocessen: en sammenligning af den Video mikroskop-baseret skrabespil sår Assay og Boyden kammer Assay

Article DOI: 10.3791/56337-v • 11:20 min • November 17th, 2017

November 17th, 2017 •

Jean-Baptiste Guy*^1,2, Sophie Espenel*^1,2, Alexis Vallard^1,2, Priscillia Battiston-Montagne¹, Anne-Sophie Wozny^1,3, Dominique Ardail^1,3, Gersende Alphonse^1,3, Chloé Rancoule^1,2, Claire Rodriguez-Lafrasse^1,3, Nicolas Magne^1,2

¹UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, ²Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, ³Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud

* These authors contributed equally

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Denne undersøgelse rapporter to forskellige metoder til analyse af celle invasion og migration: Boyden kammer assay og in vitro- video mikroskop-baserede sårheling analysen. Protokoller for disse to eksperimenter er beskrevet, og deres fordele og ulemper sammenlignes.

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Kræftforskning sag 129 Boyden kammer Scratch sår Invasion/migration celle motilitet Video-mikroskopi celle chemotaxis

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