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Journal / Biochemistry / 基于蓝光照射诱发甲沉淀的 DNA…
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Biochemistry
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JoVE Journal Biochemistry
基于蓝光照射诱发甲沉淀的 DNA 染色方法
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基于蓝光照射诱发甲沉淀的 DNA 染色方法

Article DOI: 10.3791/56528-v • 08:12 min • January 28th, 2018
January 28th, 2018 •
1, 1, 1
1Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile

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这种方法允许选择性染色和量化的 DNA 在凝胶中浸泡在 SYBR 绿 I/硝基蓝唑溶液, 然后暴露在阳光或蓝光光源。这会产生明显的沉淀, 几乎不需要任何设备, 这使得它非常适合于现场使用。

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生物化学 问题 131 脱氧核糖核酸染色 脱氧核糖核酸完整性 脱氧核糖核酸定量 SYBR 绿色 甲 硝基蓝色唑 蓝色光
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