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Melhorado o hidrogel 3D culturas de células gliais primárias para In Vitro modelagem de Neuroinflammation
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1Department of Psychiatry,University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART),University of Alberta, 3Department of Chemical and Materials Engineering,University of Alberta, 4Division of Physical Medicine and Rehabilitation,University of Alberta, 5Centre for Neuroscience,University of Alberta
Kapitel
- 00:05Titel
- 00:43Coverslip and Mold Preparation
- 02:27Preparation of Cells for Encapsulation
- 04:38Cell Encapsulation
- 06:58Results: Confocal Microscopy and 2D and 3D Mixed Cultures
- 08:47Conclusion
Neste documento, apresentamos um protocolo para a cultura 3D do rato as células da glia derivado do cérebro, incluindo astrócitos, oligodendrócitos e micróglia. Vamos demonstrar a cultura de células primárias, síntese de hidrogel de ácido hialurônico methacrylated (HAMA), encapsulamento HAMAphoto-polimerização e celular e amostra de processamento de imagem microscópica confocal e varredura de elétrons.
