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Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
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1Department of Psychiatry,University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART),University of Alberta, 3Department of Chemical and Materials Engineering,University of Alberta, 4Division of Physical Medicine and Rehabilitation,University of Alberta, 5Centre for Neuroscience,University of Alberta
Chapters
- 00:05Title
- 00:43Coverslip and Mold Preparation
- 02:27Preparation of Cells for Encapsulation
- 04:38Cell Encapsulation
- 06:58Results: Confocal Microscopy and 2D and 3D Mixed Cultures
- 08:47Conclusion
Herein, we present a protocol for the 3D culture of rat brain-derived glia cells, including astrocytes, microglia, and oligodendrocytes. We demonstrate primary cell culture, methacrylated hyaluronic acid (HAMA) hydrogel synthesis, HAMAphoto-polymerization and cell encapsulation, and sample processing for confocal and scanning electron microscopic imaging.
