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Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
Improved 3D Hydrogel Cultures of Primary Glial Cells for <em>In Vitro</em> Modelling of Neuroinflammation
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

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09:19 min

December 08, 2017

DOI:

09:19 min
December 08, 2017

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Summary

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Herein, we present a protocol for the 3D culture of rat brain-derived glia cells, including astrocytes, microglia, and oligodendrocytes. We demonstrate primary cell culture, methacrylated hyaluronic acid (HAMA) hydrogel synthesis, HAMAphoto-polymerization and cell encapsulation, and sample processing for confocal and scanning electron microscopic imaging.

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