Improved 3D Hydrogel Cultures of Primary Glial Cells for <em>In Vitro</em> Modelling of Neuroinflammation

Kyle M. Koss; Matthew A. Churchward; Andrea F. Jeffery; Vivian K. Mushahwar; Anastasia L. Elias; Kathryn G. Todd

doi:10.3791/56615

Journal

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Bioengineering

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Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Improved 3D Hydrogel Cultures of Primary Glial Cells for <em>In Vitro</em> Modelling of Neuroinflammation

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

14,633 Views

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09:19 min

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December 08, 2017

DOI:

10.3791/56615-v

DOI:

10.3791/56615-v

•

09:19 min

December 08, 2017

•

14597 Views

Kyle M. Koss^1,2, Matthew A. Churchward^1,2, Andrea F. Jeffery^1,3, Vivian K. Mushahwar^1,2,4, Anastasia L. Elias³, Kathryn G. Todd^1,2,5

¹Department of Psychiatry,University of Alberta ²Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART),University of Alberta ³Department of Chemical and Materials Engineering,University of Alberta ⁴Division of Physical Medicine and Rehabilitation,University of Alberta ⁵Centre for Neuroscience,University of Alberta

Summary

Herein, we present a protocol for the 3D culture of rat brain-derived glia cells, including astrocytes, microglia, and oligodendrocytes. We demonstrate primary cell culture, methacrylated hyaluronic acid (HAMA) hydrogel synthesis, HAMAphoto-polymerization and cell encapsulation, and sample processing for confocal and scanning electron microscopic imaging.