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Journal / Genetics / 单液滴数字聚合酶链反应对热点地区突变的综合和同步检测…
JoVE Journal
Genetics

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单液滴数字聚合酶链反应对热点地区突变的综合和同步检测
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单液滴数字聚合酶链反应对热点地区突变的综合和同步检测

Article DOI: 10.3791/58051-v • 08:23 min • September 25th, 2018
September 25th, 2018 •
1,2, 1, 1, 1,3,4, 1,3,5, 1,6, 1
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, 2CNRS UMR144, Institut Curie, PSL Research University, 3Department of Medical Oncology, Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830, Institut Curie, PSL Research University

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Summary
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在这里, 我们提出了一个协议, 以准确地量化一个目标区域的多基因变化, 在单一的反应使用下拉 ddPCR 和一个独特的对水解探针。

Tags

遗传学 问题 139 脱落 ddPCR 热点突变 癌症 循环肿瘤 DNA 诊断
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