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Journal / Developmental Biology / Insan pluripotent kök hücrelerinin karaciğer…
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Developmental Biology

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Insan pluripotent kök hücrelerinin karaciğer hücrelerine verimli farklılaşma

Article DOI: 10.3791/58975-v • 07:37 min • June 11th, 2019
June 11th, 2019 •
1,2, 1, 1
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute, Stanford University School of Medicine, 2Department of Developmental Biology, Stanford University School of Medicine

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Bu protokol, 18 gün içinde insan pluripotent kök hücrelerinden (hPSCs) hepatosit benzeri hücreleri verimli bir şekilde üretmek için bir monolayer, serum içermeyen bir yöntem ayrıntıları. Bu hpscs sırayla ilkel çizgi, kesin endoderm, posterior foregut ve karaciğer tomurcuk ataları hepatosit benzeri hücreler oluşturmadan önce gibi ara hücre türlerine ayırt olarak altı adım gerektirir.

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Gelişimsel biyoloji Sayı 148 Insan pluripotent kök hücresi verimli farklılaşma endoderm karaciğer progenitor hepatosit
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