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Journal / Biochemistry / 通过后染色方法在聚丙烯酰胺凝胶中对芒果标记RNA的荧光可视化…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
通过后染色方法在聚丙烯酰胺凝胶中对芒果标记RNA的荧光可视化
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Journal (Biochemistry)
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
Journal (Biochemistry)
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
 
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通过后染色方法在聚丙烯酰胺凝胶中对芒果标记RNA的荧光可视化

Article DOI: 10.3791/59112-v • 06:06 min • June 21st, 2019
June 21st, 2019 •
*1, *1, 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University
* These authors contributed equally

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Summary
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在这里,我们提出了一种敏感、快速和鉴别的凝胶后染色方法,使用原生或变性聚丙烯酰胺凝胶电泳(PAGE)凝胶(PAGE)凝胶,将带有RNA芒果贴片I、II、III或IV标记的RNA成像。运行标准 PAGE 凝胶后,芒果标记的 RNA 可轻松与 TO1-Biotin 染色,然后使用常用的荧光读卡器进行分析。

Tags

生物化学 问题 148 RNA 芒果 荧光 变性凝胶 原生凝胶 凝胶染色 检测方法 PAGE
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