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1Department of Geology & Geophysics,Yale University, 2Department of Ecology & Evolution,Stony Brook University, 3Neurogenetics of Vocal Communication,Max Planck Institute for Psycholinguistics, 4School of Biological and Chemical Sciences,Queen Mary University of London, 5School of Biology & Environmental Science,University College Dublin, 6Donders Institute for Brain, Cognition and Behavior, 7Consortium for Inter-Disciplinary Environmental Research,Stony Brook University
Kapitel
- 00:04Titel
- 00:52Cranial Dissections for RNA
- 04:05Postcranial Dissections for RNA
- 06:24Tissue Culture
- 09:41Refreshing Media and Passaging Cells
- 11:38Conclusion
- 13:15Freezing Viable Living Cells and Thawing the Frozen Cells
- 14:48Results: Tissue Preparation and Cell Culture Results
Questo è un protocollo per la preparazione ottimale dei tessuti per analisi genomiche, trascrittomiche e proteomiche di pipistrelli catturati in natura. Esso comprende protocolli per la cattura pipistrello e la dissezione, la conservazione dei tessuti, e la coltura cellulare del tessuto pipistrello.
